Testing notes for in vitro data for the project: "Reference Models for Multi-Layer Tissue Structures". Protocol deviations, data collection notes, and thickness analysis voids/notes are included for each subject. Notes captured from https://simtk.org/plugins/moinmoin/multis/InVitroExperiments?action=recall&rev=40. A dynamic version of the testing notes can be found at https://simtk.org/plugins/moinmoin/multis/InVitroExperiments. #acl +All:read Default #format wiki #language en <> === CMULTIS001-1 === ''mock'' * No Optorak collected, software under development === CMULTIS002-1 === ''mock'' . '''Extremity''' : Leg * Optotrak output incorrectly indexed, not all data stored === CMULTIS002-2 === ''mock'' . '''Extremity''' : Leg * Optotrak data correctly corrected === CMULTIS002-3 === ''mock'' . '''Extremity''' : Arm * Humerus sensor file required debugging * Firewall on Tyler's account prevented Optotrak connection, another was able to establish a connection === CMULTIS003-1 === ''mock'' . '''Extremity''' : Arm * Optotrak triax is thought to have come loose, skewing relative probe position for 4 positions in lower arm. === CMULTIS004-1 === . '''Extremity''' : Leg * Optotrak attachment plugs modified to receive #4-1 zinc plated screws instead of #3-3/4 brass screws to strengthen triax connection. * Posterior lower leg ultrasound targeted fibula instead of tibia * A new technique was developed to assist in determining lower tissue boundary in order to differentiate bone layers from tissue layers. By palpating the skin while viewing ultrasound, it is possible to see the compression of tissue or non-compression of bone. * Data Analysis * F6 marker digitizing is poor, so only used F1-F5 for registration to the imaging data * Trial 017 (Lower Leg Anterior Distal): Missing bone boundary for MR slice and CT slice images (R01 registration). Moved image plane slightly, within ultrasound probe thickness until bone boundary was visible prior to analysis. * Preliminary thickness comparison [[attachment:CMULTIS004-1_CTSlice_US_comparison.pdf]] . '''Extremity''' : Arm * All digitized points for humerus registration markers stored as identical coordinates. * The optotrak cluster attached to the lower arm was not visible during posterior lower arm trials although no error messages were reported. * An existing analysis script is being modified to check all collected data after each trial on the data collection computer. * A retest of lower arm is scheduled for this Wednesday the 6/7/17. * The optotrak markers will be placed in the exact same location, using the same screw holes if possible. * If optotrak makers can be placed in same location, the lower arm registration will be compared from the first set to the new set, and if little difference is detected, the new upper arm registration data may be used . '''RETEST''': Arm * The adapted analysis script was successfully used to check data immediately following the experiment. All data was stored. * Data Analysis * All radius digitized spheres were stored in the proximal positions. Modified data analysis script to name the radius markers correctly. * Trial 100 (Lower Arm Medial Proximal): Missing bone boundary for MR slice image (R01 registration). Moved image plane slightly, within ultrasound probe thickness until bone boundary was visible prior to analysis. === CMULTIS005-1 === . '''Extremity''' : Leg * No protocol deviation * Data Analysis * Trial 020 (Lower Leg Medial Central): Missing bone boundary for MR slice image (R01 registration). Moved image plane slightly, within ultrasound probe thickness until bone boundary was visible prior to analysis. . '''Extremity''' : Arm * No protocol deviation * Data Analysis * Trial 042 (Lower Arm Lateral Proximal): Missing bone boundary for MR slice image (R01 registration). Moved image plane slightly, within ultrasound probe thickness until bone boundary was visible prior to analysis. === CMULTIS006-1 === . '''Extremity''' : Leg * No protocol deviation . '''Extremity''' : Arm * The 9L4 ultrasound probe was used due to large size of specimen === CMULTIS007-1 === . '''Extremity''' : Leg * Possibly thawed previously * NDI First Principles failed to connect half way through the experiment. A settings file had to be removed fro the ndigital folder, software ran, settings file restored, software ran again. * The US probe had to be re digitized, which does not affect prior successful runs. This was verified by running the analysis script. * Data Analysis * Trial 029 (Lower Leg Anterior Proximal), Trial 037 (Lower Leg Medial Proximal), Trial 035 (Lower Leg Lateral Central), Trial 030 (Lower Leg Anterior Central): Missing bone boundary for MR slice image (R01 registration). Moved image plane slightly, within ultrasound probe thickness until bone boundary was visible prior to analysis. . '''Extremity''' : Arm * No protocol deviation === CMULTIS008-1 === . '''Extremity''' : Leg * No protocol deviation . '''Extremity''' : Arm * No protocol deviation * Data Analysis * Trial 052 (Lower Arm Medial Distal), Trial 063 (Lower Arm Posterior Distal): Missing bone boundary for MR and CT slice images (R01 registration). Moved image plane slightly, within ultrasound probe thickness until bone boundary was visible prior to analysis. === CMULTIS009-1 === . '''Extremity''' : Leg * No protocol deviation * Data Analysis * F5 marker digitizing is poor, so only used F1-F4 and F6 for registration to the imaging data. Modified data analysis script to remove T5. . '''Extremity''' : Arm * No protocol deviation === CMULTIS010-1 === . '''Extremity''' : Leg * No protocol deviation * Data Analysis * Trial 068 (Lower Leg Posterior Distal): Missing bone boundary for CT slice image (R01 registration). Moved image plane slightly, within ultrasound probe thickness until bone boundary was visible prior to analysis. . '''Extremity''' : Arm * No protocol deviation * Data Analysis * Trial 021 (Lower Arm Medial Distal): Missing bone boundary for CT slice image (R01 registration). Moved image plane slightly, within ultrasound probe thickness until bone boundary was visible prior to analysis. === CMULTIS011-1 === . '''Extremity''' : Leg * All experimentation performed on one day. Therefor the specimen was thawed for less total time and has one less freeze/thaw cycle (surgical tools done same day too) * MRI imaging: The imaging window was not large enough for a portion of the upper leg, therefore the skin and fat thickness is not captured for a portion of the proximal lateral side. * Data Analysis * T6 marker digitizing is poor, so only used T1-T5 for registration to the imaging data. Modified data analysis script to remove T6. * Trial 55 (Lower Arm Medial Distal): Missing bone boundary for CT slice and MR slice images (R01 registration). Moved image plane slightly, within ultrasound probe thickness until bone boundary was visible prior to analysis. === CMULTIS012-1 === . '''Extremity''' : Leg * Completed over the course of two days. One less freeze/thaw cycle due to surgical tools completion on day two. * Data Analysis * T6 marker digitizing is poor, so only used T1-T5 for registration to the imaging data. Modified data analysis script to remove T6.