Testing notes for surgical tools data for the project: "Reference Models for Multi-Layer Tissue Structures". Protocol deviations, data collection notes, and thickness analysis voids/notes are included for each subject. Notes captured from https://simtk.org/plugins/moinmoin/multis/InstToolsExperiments?action=recall&rev=22. A dynamic version of the testing notes can be found at https://simtk.org/plugins/moinmoin/multis/InstToolsExperiments. MRI and CT image files (.nii) associated with each subject are included on the sister website (https://multisgamma.stanford.edu). The numbering system between subjects in surgical tools and in vitro experiments is the same (for non-mock trials): CMULTIS005-1 is the same as SMULTIS005-1. === SMULTIS001-1 mock === donor: CMULTIS002 * First mock of instrumented surgical tool procedures minus Vic3D strain measurement. 1. Cut skin 10 cm - measured length, width, depth 1. retracted skin 2 cm 1. widened incision with forceps 1. closed incision for approximation with forceps 1. everted tissue with forceps 1. (trial #16) closed suture with square knot at midpoint connected to scalpel bolt 1. (17) closed suture with a loop, no knot, take first trial not last. 1. (18) closed suture with surgeons knot (first knot) 1. (19) tied 3rd knot (second was not instrumented) 1. (20 & 21) tissue pulling. Not a loop like (17). tied knot to one end, pulled on other. mechanically the same as the loop, just pulled on one side instead of both. * Notes: * finalize trial naming convention * build in length, width, depth window after each trial * build apparatus to hold metal ruler (use manfrotto arm) === SMULTIS001-2 mock === donor: CMULTIS002 (the name was actually SMULTIS002-1 but this will be archived as mock data to leave 002 for a different donor) '''Goal''': Test Vic3D system integration and use during first three surgical procedures * all software and hardware modifications were made prior * Vic3D triggered at 30fps for 20s by multis computer * Synchronization signal also sent and stored with Vic3D * For each trial, a folder was created for Vic3D * An ink roller was used and worked seemingly well * Different camera angles were tested for optimal viewing and minimal tool shadowing * Notes for next time * Create calibration folder for the day's experiment * use for all trials that day * tilt cameras towards operator * move cameras closer together to reduce shadowing effect === SMULTIS003-1 mock === donor: CMULTIS003 '''Goal''': Full data collection including strain measurement Notes: * although DIC can only measure 4-5cm cut length, make cut full length. DIC will occur on first half, so start cut at beginning of DIC region, continue 5cm after end of DIC * do not retract after skin cut with current retractor because it is too large relative to the cut depth (0.6mm) * move the forceps wid/eve/clos and suture steps to after the cut through skin fat interface so that more tissue is available. The forceps will essentially be grabbing the skin plus skin/fat interface * for retraction, start at midline and continue to retract 1cm after contact * subtract ruler thickness from caliper depth measurement {{attachment:skin.png||width="300"}} === SMULTIS004-1 === donor: CMULTIS004 date: 08-23-18 '''Goal''': First non-mock experiment '''Protocol Deviations''' * Specimen underwent 1 extra freeze thaw cycle due to software malfunction on first attempted experiment * To reduce reflection during indentation the polarized VIC3d light filters were first optimized to the undeformed skin, then rotated 90 degrees. This method will be used from now on. * A shadow not visible to the naked eye blocked VIC3d strain measurement of the cut trial. A mock cut will be performed from now on. * Extra force/moment data was collected at the beginning of each trial. A series of tap tests were conducted to show that the extra data is exactly the length of the difference between the force data time (~13s) and optotrak data time (~10s). The last 10s of each optotrak and force are synchronized by removing that difference. === SMULTIS005-1 === donor: CMULTIS005 date: 09-14-18 '''Protocol Deviations''' * None === SMULTIS006-1 === donor: CMULTIS006 date: 09-21-18 '''Protocol Deviations''' * None === SMULTIS007-1 === donor: CMULTIS007 date: 10-02-18 '''Protocol Deviations''' * White ink used successfully for dark skin === SMULTIS008-1 === donor: CMULTIS008 date: 10-05-18 '''Protocol Deviations''' * White ink used successfully for dark skin === SMULTIS009-1 === donor: CMULTIS009 date: 10-17-18 '''Protocol Deviations''' * SXX_CUT_FAT * Just over half the length of the cut penetrated the muscle fascia below * SXX_CUT_FMI was therefore not possible and not collected following the SXX_CUT_FAT trial. * The remaining fascia and roughly 3mm (depth) of muscle was cut but not recorded to prepare the tissue for the muscle retraction trial. '''Notes''' * Possibly best VIC3D yet for each of the three trials === SMULTIS010-1 === donor: CMULTIS010 date: 10-18-18 '''Notes''' * VIC3D results for all three trials comparable to 009. * Tissue recovered atypically well during indentation, likely due to excess and relatively young subcutaneous tissue === SMULTIS011-1 === donor: CMULTIS011 date: 10-30-18 '''Notes''' * VIC3D cut terial was not the best. Tissue was not dark or light. Both white and black speckle was attempted * This specimen underwent one less freeze-thaw cycle and was kept thawed one day less than others. The Ultrasound and Surgical Tools and CT/MRI were all completed in one day. === SMULTIS012-1 === donor: CMULTIS012-1 date: 11-06-18 * Very good VIC3D trials. * Missing fat cut trial because of extremely thin fat layer.